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上海小鼠血管紧张素Ⅱ试剂盒说明书

  • 型号:Wk20081价格: 电议
  • 产地:美国 点击量:2591

产品简介: 产品名称:上海小鼠血管紧张素Ⅱ试剂盒说明书联系电话:021—60449724,13296089724Specimenrequirements1. extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,andshouldbeexperimentassoon

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产品名称:上海小鼠血管紧张素Ⅱ试剂盒说明书
联系电话:021—60449724,13296089724
Specimen requirements
1. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1. Dilute and add sample:Dilute Original density Standard as follow table:
20μg/L 5 Standard 150μl Original density Standard+150μl Standard diluent
10μg/L 4 Standard 150μl 5 Standard+150μl Standard diluent
5μg/L 3 Standard 150μl 4 Standard+150μl Standard diluent
2.5μg/L 2 Standard 150μl 3 Standard +150μl Standard diluent
1.25μg/L 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except  blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
上海小鼠血管紧张素Ⅱ试剂盒说明书”上海劲马生物现货供应,购买立减500元,提供代测服务,产品范围: 人,小大鼠,猪,兔,其它动物细胞因子;细胞凋亡,活性多肽,自身抗体 ,血栓与止血, 骨代谢,肝纤维化,肿瘤,激素内分泌,自身抗体科研ELISA检测试剂盒等。

原创作者:上海劲马实验设备有限公司

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