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上海人基质细胞衍生因子1a试剂盒

  • 型号:Wk10072价格: 电议
  • 产地:美国 点击量:2501

产品简介: 产品名称:上海人基质细胞衍生因子1a试剂盒操作步骤:参照说明书灵敏度:具体请查看说明书.检测范围(RANGN):具体请查看说明书.参考值:产品用途:科研ELISA检测试剂盒.提供实验代测服务.您只需将需要检测的种属(Human,Rat,Mouse,Rabbit,Monkey,Pig……)和检测指标(介素类、因子类)及标本数量(48

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产品详细

产品名称:上海人基质细胞衍生因子1a试剂盒
操作步骤:
参照说明书
灵敏度:
具体请查看说明书.
检测范围(RANGN):
具体请查看说明书.
参考值:
产品用途:
科研ELISA检测试剂盒.提供实验代测服务.您只需将需要检测的种属(Human,Rat,Mouse,Rabbit,Monkey, Pig……)和检测指标(介素类、因子类)及标本数量(48T/96T)通知公司业务员即可。在接到客户标本当日起,现货产品一周内将检测报告交到客户手中!
产品范围:
人,小鼠,大鼠,猪,兔,其它动物细胞因子;细胞凋亡,活性多肽、自身抗体 ,血栓与止血,
骨代谢,肝纤维化,肿瘤,激素内分泌、自身抗体科研ELISA检测试剂盒

试剂的准备 

1.   标准品:标准品的系列稀释应在实验时准备,不能储存。稀释前将标准品振荡混匀。稀释比例按下表中进行:

1000

pg/ml

6号标准品)

原倍浓度不用稀释直接加入50ul

500

pg/ml

5号标准品)

100ul的原倍标准品加入100ul的标准品稀释液

250

pg/ml

4号标准品)

100ul5号标准品加入100ul的标准品稀释液

125

pg/ml

3号标准品)

100ul4号标准品加入100ul的标准品稀释液

62.5

pg/ml

2号标准品)

100ul3号标准品加入100ul的标准品稀释液

31.2

pg/ml

1号标准品)

100ul2号标准品加入100ul的标准品稀释液

0

pg/ml

(空白对照)

原倍浓度不用稀释直接加入50ul

 2.   洗涤缓冲液(50×)的稀释:蒸馏水50倍稀释。 

试剂盒性能

 1.   灵敏度:最小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990

2.   特异性:不与其它细胞因子反应。

3.   重复性:板内、板间变异系数均小于10%

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Storage and validity
1.Storage:  2-8℃.
2.validity: six months.

原创作者:上海劲马实验设备有限公司

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