产品名称：人乙型肝炎表面抗原试剂盒价格产品包装：48T/96T
作用：科研使用
PROCEDURAL NOTES/LAB.QUALITY CONTROL
?When not in use, kit components should be stored refrigerated or frozen as indicated on vials or bottles. All reagents should be warmed to room temperature before use. Lyophilized standards should be discarded after use.
?Once the desired number of strips has been removed, immediately reseal the bag to protect the remaining strips from edterioration.
?Cover or cap all reagents when not in use.
?Do not mis or interchange reagents between different lots.
?Do not use reagents beyond the expiration date of the kit .
?Use a clean disposable plastic pipette tip for each reagent, standard, or specimen addition in order to avoid cross-contamination, for the dispensing of H2SO4 and substrate solution, avoid pipettes with metal parts.
?Use a clean plastic container to prepare the washing solution.
?Thoroughly mix the reagents and samples before use by agitation or swirling.
?All residual washing liquid must be drained from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
?The TMB solution is light sensitive. Avoid prolonged exposure to light, also, avoid contact of the TMB solution with metal to prevent colour development. Warning TMB is toxic avoid direct contact with hands. Dispose off properly. If a dark blue colour develops within a few minutes after preparation, this indicates that the TMB solution has been contaminated and must be discarede. Read absorbances within 1 hour after completion of the assay.
?When pipetting reagents, maintain a consistent order of addition from well-to-well. This will ensure equal incubation times for all wells.
?Respect incubation times described in the assay procedure.
操作注意事项
1．试剂应按标签说明书储存，使用前恢复到室温。稀稀过后的标准品应丢弃，不可保存。
2．实验中不用的板条应立即放回包装袋中，密封保存，以免变质。
3．不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4．使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。
5．使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6．洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。
7．底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。
8．加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。
9．按照说明书中标明的时间、加液的量及顺序进行温育操作。
人乙型肝炎表面抗原试剂盒特点：1.准确度高，灵敏度高，特异性强2.检测时间短3.操作简便4.安全可靠;含税含运费，试剂盒包装为：48T/96T，48T可以测42样,96T可以测84样，提供免费代测及技术指导，欢迎咨询。
原创作者：上海劲马实验设备有限公司
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